Expression data from Triple Negative Breast Cancer (TNBC) cell lines cultured in 2D and 3D.. Expression data from Triple Negative Breast Cancer (TNBC) cell lines cultured in 2D and 3D.

Development of a reliable method for human triple-negative breast cancer organotypic culture: Improving imaging and genomic studies in 3D cultures The primary objectives of this study are to develop an advanced three-dimensional cell culture system to better model the tumor microenvironment in triple-negative breast cancer (TNBC), analyze the differences in molecular and cellular behavior between two-dimensional (2D) and 3D cultures, and investigate the impact of these differences on key oncogenic signaling pathways, specifically PI3K and β-catenin. Overall design: For RNA extraction and hybridization on Human Clariom D Affymetrix microarrays. 10 experiments of TNBC cell lines were used; 6 in 2D cell cultured and 4 in 3D cell culture using the Solid Hanging drop method.