Non-specificity of inhibitions by BzBr.
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The relative percentage of hydroxylated product formed is shown in comparison to the positive controls. In each CYP column, first sub-column is without any inhibitor, second and third sub-columns are with an equivalent amount of BzBr and benzothiadiazole respectively. Concentrations of baculosomes- 20 nM CYP, 250 µM NADPH and 80 µM Diclof or pNP. Sampling for hydroxylated product analysis was at 30 minutes. [*nd: product formed was too low to be detected under the reaction assay conditions].
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2015-12-02
