Bisulfite-sequencing of LX-2 cells

Liver fibrosis is characterized by deposition of excessive extracellular matrix (ECM). The major source of ECM is activated hepatic stellate cells (HSCs).NSUN2 is one of the m5C methyltransferases, mainly responsible for the methylation of tRNA and mRNA and plays a significant role in RNA m5C methylation. The purpose of this Bisulfite-seq is to explore the role of NSUN2 acting as m5C methyltransferases during HSC activation. Overall design: The changes of m5C modification in LX-2 cells after TGF-ß1 stimulated were investigated by Bisulfite-sequencing.