Tau phosphorylation sites identified in sod2 null mouse brain tissue

a: Percent changes of listed residue in sod2 null mice relative to sod2wild-types (normalized to 100%, see Figure 1), both treated with EUK189 at 1 mg/kg quantitated by Western blotting with monoclonal antibodies for the indicated tau residues, NS– not significant, ND – not done.b: identifications were positive (+) if a MS/MS spectrum could be unambiguously interpreted to identify a unique phosphorylation site. In three cases (+/−) assignments were based on MS or MS/MS data and alternative sites were possible.c: several matching phospho peptides observed by ESI-MS (i.e., peptides: mono-/di-phosph. S195-R209 and mono-/di-phosph. S181-R209);d: Phosphorylate site possibly on Ser-404, Thr-403 or Ser-400;e: peptide observed with 1P and 2P groups;f: differences in peptide sequence (human vs. mouse) likely accounting for negative antibody result (homologous human peptide: TPPAPKTPPSSGEPPK);g: no antibody available for Ser-159.