Next Generation Sequencing Facilitates Quantitative Analysis of mRNA lifetime of Wild Type and Mettl3-/- mice

Considering m6A modification in mRNA was reported to be intensively correlated with RNA decay, for example, T cells and neurone cells. We wondered whether deleting Mettl3, the main catalzing enzyme, affect the RNA stability of mouse dendritic cells. Two sets representing WT and KO dendritic cells were analyzed, each set contains three samples indicating three different treatment with Actinomycotoxin D for 0, 3, or 6h. No replicate was included.