A map of protein-metabolite interactions reveals principles of chemical communication

Application of a novel mass spectrometry-based high-throughput workflow (LiP-SMap) and data resource based on limited proteolysis (LiP) of complex samples. Proteome-wide limited proteolysis sites were obtained in different conditions to infer ligand-induced and protein-protein interaction induced conformational changes. This information was used to identify unknown ligand binding proteins, small molecule-protein binding sites and the remodeling of protein complexes directly in cells.