Identification of RNA molecules bound on IIBs of polyQ-expanded ATXN1
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https://www.ncbi.nlm.nih.gov/sra/ERP149827
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SH-SY5Y cells overexpressing EYFP-ATXN1(Q82) under the control of the Tet-On promoter were generated using the Sleeping Beauty Transposon. These cells accumulate insoluble IIBs of polyQ-expanded ATXN1. In order to determine the identity of the RNA molecules bound on insoluble polyQ IIBs, RNA was isolated fro YFP-ATXN1(Q82) IIBs, followed by RNA-sequencing. RNA was also isolated and sequenced from control samples (CTRL) which did not contain any inclusions.
创建时间:
2023-09-23



