RNA-Seq of liver in milk fat depression dairy cows

Milk fat depression (MFD) was usually caused by feeding high energy diet to lactating cows. Eight primiparous Holstein cows were randomly assigned to sequences in a 2*2 crossover treatment design. During the induction period, the cows were fed with either a control diet or a high-starch and high-oil diet, and all the cows were fed the control diet during the subsequent washout period. Milk and liver samples were collected, and total RNAs were isolated and sequenced by RNA-seq.