We develop a CRISPR-Cas assisted random mutation (CARM) technology for whole genome mutagenesis. As a proof-of-principle, CARM was applied to evolve the capacity of Saccharomyces cerevisiae to produce
The construction of powerful cell factories requires intensive and extensive remodelling of microbial genomes. Considering the rapidly increasing number of these synthetic biology endeavours, there is
This archive contains the optimized plqH genes used in the study "Heterologous plastoquinone production using a newly identified O2-dependent cyanobacterial hydroxylase", submitted to The FE
