Single_cell_analysis_of_the_transcriptomic_heterogeneity_of_ground_state_pluripotency_____

This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes, e.g. Nanog, in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study, we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum/LIF and 2i/LIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.