Appendix III. Molecular data of the genus Brachycephalus used in the present study. Samples included in the phylogenetic inferences, 16 S sequence accession number and sample metadata. * Holotype. in A new species of flea-toad of the genus Brachycephalus Fitzinger, 1826 (Anura: Brachycephalidae) from the cloud forests of Serra do Mar, southeastern Brazil

Appendix III. Molecular data of the genus Brachycephalus used in the present study. Samples included in the phylogenetic inferences, 16S sequence accession number and sample metadata.* Holotype. SpecieSpecimen-VoucherGenBank Acc. numberBody planSpecies groupLocalBrachycephalus nanicus sp. novZUEC-AMP25581OR250404leptodactyliformB. puriBananal, SPBrachycephalus nanicus sp. novZUEC-AMP25578OR250405leptodactyliformB. puriBananal, SPBrachycephalus nanicus sp. novZUEC-AMP25577OR399156leptodactyliformB. puriBananal, SPBrachycephalus nanicus sp. novZUEC-AMP25580OR399157leptodactyliformB. puriBananal, SPBrachycephalus puriMZUSP139152OK298954leptodactyliformB. puriIbitirama, ESBrachycephalus puriMZUSP139154OK298955leptodactyliformB. puriIbitirama, ESBrachycephalus sp8CTMZ 01522/ MZUSP133731MK697410leptodactyliformB. puriBananal, SPBrachycephalus sp8HQ435692leptodactyliformB. puriIlha Grande, Angra dos Reis, RJBrachycephalus pulexPU65MT929509leptodactyliformCamacan, BABrachycephalus pulexMZ770742leptodactyliformArataca, BABrachycephalus dacnisZUEC-AMP 24982OR250414leptodactyliformReserva Dacnis, Ubatuba, SPBrachycephalus dacnisZUEC-AMP 25270OR250415leptodactyliformReserva Dacnis, Ubatuba, SPBrachycephalus hermogenesiTC129MT929506leptodactyliformSalesópolis, SPBrachycephalus hermogenesiTC133MT929507leptodactyliformSalesópolis, SPBrachycephalus hermogenesiMVTIS 3210MZ770740leptodactyliformSão Luiz do Paraitinga, SPBrachycephalus hermogenesiZUEC-AMP 24983OR250409leptodactyliformReserva Dacnis, Ubatuba, SPBrachycephalus hermogenesiZUEC-AMP 24980OR250410leptodactyliformReserva Dacnis, Ubatuba, SPBrachycephalus didactylusCFBH11506MK697365/ JX267467leptodactyliformTerespolis, RJBrachycephalus didactylusMNRJ37327MT929504leptodactyliformTerespolis, RJBrachycephalus sulfuratusCFBH39141MK697392leptodactyliformSão Francisco do Sul, SCBrachycephalus sulfuratusMZ770751leptodactyliformSão Francisco do Sul, SCBrachycephalus clarissaeMNRJ 56504MK697394intermediateNova Friburgo, RJBrachycephalus clarissaeMNRJ 56516MK697395intermediateNova Friburgo, RJBrachycephalus darksidePPGT600MT734440bufoniformB. ephippiumErvalia, MGBrachycephalus ephippiumMNRJ 56517MK697366bufoniformB. ephippiumCachoeiras de Macacu, RJBrachycephalus ephippiumCFBH 12355MK697367bufoniformB. ephippiumTerespolis, RJBrachycephalus garbeanusMNRJ51472MT929466bufoniformB. ephippiumMacaé de Cima, RJBrachycephalus garbeanusCFBH16800MT929467bufoniformB. ephippiumMacaé de Cima, RJBrachycephalus ibitingaCFBH 19348MK697399bufoniformB. ephippiumItanhaem, SPBrachycephalus ibitingaCFBH 2978MK697401bufoniformB. ephippiumSão Paulo, SPBrachycephalus margaritatusMNRJ73702MT929478bufoniformB. ephippiumEngenheiro Paulo de Frontin, RJBrachycephalus margaritatusMNRJ76100MT929479bufoniformB. ephippiumEngenheiro Paulo de Frontin, RJBrachycephalus rotenbergaeCFBH16828MT929457bufoniformB. ephippiumSão José dos Campos, SPBrachycephalus rotenbergaeMVTIS 3213MZ770745bufoniformB. ephippiumMogi das Cruzes, SPBrachycephalus sp6CFBH 44422MK697406bufoniformB. ephippiumBananal, SPBrachycephalus sp6CFBH 44424MK697407bufoniformB. ephippiumCunha, SPBrachycephalus alipioiHQ435690bufoniformB. vertebralisVargem Alta, ESBrachycephalus alipioiAL1MT929481bufoniformB. vertebralisVargem Alta, ESBrachycephalus bufunoidesZUFRJ 15428MH259788bufoniformB. vertebralisNova Friburgo, RJBrachycephalus bufunoidesZUFRJ 15426MH259791bufoniformB. vertebralisNova Friburgo, RJBrachycephalus crispusTC34MT929483bufoniformB. vertebralisCunha, SPBrachycephalus crispusTC36MT929484bufoniformB. vertebralisCunha, SPBrachycephalus guaraniPRU1MT929485bufoniformB. vertebralisUbatuba, SPBrachycephalus guaraniPRU3MT929487bufoniformB. vertebralisUbatuba, SPBrachycephalus herculeusCFBH13729OQ629303bufoniformB. ephippiumSanta Maria Madalena, RJBrachycephalus herculeusCFBH13730OQ629304bufoniformB. ephippiumSanta Maria Madalena, RJBrachycephalus nodotergaBO130KJ649772bufoniformB. vertebralisSalespolis, SPBrachycephalus nodotergaCA2226KJ649779bufoniformB. vertebralisSerra Cantareira, São Paulo, SPBrachycephalus nodotergaIB634KJ649781bufoniformB. vertebralisSão Sebastiao, SPBrachycephalus pitangaHQ435699bufoniformB. vertebralisSão Luiz do Paraitinga, SPBrachycephalus pitangaMVTIS 3205MZ770741bufoniformB. vertebralisSão Luiz do Paraitinga, SPBrachycephalus sp3CFBH 44409MK697397bufoniformB. vertebralisUbatuba, SPBrachycephalus tobyHQ435701bufoniformB. vertebralisUbatuba, SPBrachycephalus tobyTO1MT929499bufoniformB. vertebralisUbatuba, SPBrachycephalus vertebralisHQ435702bufoniformB. vertebralisUbatuba, SPBrachycephalus vertebralisCFBH1388MT929502bufoniformB. vertebralisUbatuba, SPBrachycephalus actaeusMZ770736bufoniformB. pernixSão Francisco do Sul, SCBrachycephalus actaeusMZ770737bufoniformB. pernixSão Francisco do Sul, SCBrachycephalus albolineatusMZ770738bufoniformB. pernixJaraguá do Sul, SCBrachycephalus auroguttatusBauroguttatus2KX025355bufoniformB. pernixGaruva, SCBrachycephalus auroguttatusMZ770739bufoniformB. pernixGaruva, SCBrachycephalus boticarioBboticario1KX025370bufoniformB. pernixBlumenau, SCBrachycephalus boticarioBboticario2KX025371bufoniformB. pernixBlumenau, SCBrachycephalus brunneusHQ435691bufoniformB. pernixCampina Grande do Sul PRBrachycephalus brunneusCAPR1MT929511bufoniformB. pernixCampina Grande do Sul PRBrachycephalus coloratusInd1MT928335bufoniformB. pernixPiraquara, PRBrachycephalus coloratusInd3MT928336bufoniformB. pernixPiraquara, PRBrachycephalus curupiraBSalto4KX025381bufoniformB. pernixSão José dos Pinhais, PRBrachycephalus curupiraBSalto5KX025382bufoniformB. pernixSão José dos Pinhais, PRBrachycephalus ferruginusHQ435695bufoniformB. pernixMorretes, PRBrachycephalus ferruginusMT929515bufoniformB. pernixMorretes, PRBrachycephalus fuscolineatusBfuscolineatus1KX025296bufoniformB. pernixIlhota, SCBrachycephalus fuscolineatusBfuscolineatus2KX025297bufoniformB. pernixIlhota, SCBrachycephalus izecksohniHQ435696bufoniformB. pernixMorretes, PRBrachycephalus izecksohniTPPR1MT929518bufoniformB. pernixMorretes, PRBrachycephalus leopardusBleopardus2KX025240bufoniformB. pernixTijucas do Sul, PRBrachycephalus leopardusBleopardus3KX025241bufoniformB. pernixTijucas do Sul, PRBrachycephalus mariaeterezaeBmariaterezae2KX025344bufoniformB. pernixJoinville, SCBrachycephalus mariaeterezaeBmariaterezae3KX025345bufoniformB. pernixJoinville, SCBrachycephalus mirissimusMH136570bufoniformB. pernixMassaranduba, SCBrachycephalus olivaceusBolivaceus1KX025300bufoniformB. pernixJoinville, SCBrachycephalus olivaceusTC236MG889435bufoniformB. pernixJoinville, SCBrachycephalus pernixHQ435698bufoniformB. pernixQuatro Barras, PRBrachycephalus pernixAGPR2MT929522bufoniformB. pernixQuatro Barras, PRBrachycephalus pombaliHQ435700bufoniformB. pernixGuaratuba, PRBrachycephalus pombaliSIPR1MT929525bufoniformB. pernixGuaratuba, PRBrachycephalus quiririensisBquiririensis2KX025318bufoniformB. pernixCampo Alegre, SCBrachycephalus quiririensisMZ770743bufoniformB. pernixCampoAlegre SCBrachycephalus sp9CFBH 42815MK697411bufoniformB. pernixCorupá, SCBrachycephalus tabuleiroMNRJ93858OR116969bufoniformB. pernixSão Bonifácio, SCBrachycephalus tabuleiroUNIFESSPA112OR116970bufoniformB. pernixSão Bonifácio, SCBrachycephalus tridactylusBtridactylus2KX025388bufoniformB. pernixGuaraqueçaba, PRBrachycephalus tridactylusTRID-5MG889432bufoniformB. pernixGuaraqueçaba, PRBrachycephalus verrucosusBverrucosus9KX025234bufoniformB. pernixJoinville, SCBrachycephalus verrucosusBverrucosus10KX025235bufoniformB. pernixJoinville, SCIschnocnema henseliMZ770752Jaraguá do Sul, SC Appendix III. 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