Deletion of Vβ3+CD4+ T cells by endogenous mouse mammary tumor virus 3 prevents type 1 diabetes induction by autoreactive CD8+ T cells

To characterize B6.H2g7 Vb3+(Trbv26+) CD4+ T-cells, which are deleted by NOD-encoded Mtv3 in B6.H2g7.Idd32NOD/NOD mice, paired single-cell RNA sequencing and single-cell TCR sequencing was performed on two separate pools of B6.H2g7 CD4+ T-cells 14 days after they were adoptively transferred into two NOD.Rag1null recipient mice. 5 x 10^6 magnetic column purified CD4+ T-cells harvested from spleens of B6.H2g7 donor mice were transferred to NOD.Rag1null hosts and recovered from host spleens 14 days later by flow cytometric sorting for single cell sequencing analysis