Effect of deletion of Sisssb and Sisdbp on DNA metabolic pathways of Saccharolobus islandicus REY15A

To investigate the putative functions of the ssDNA binding proteins SisSSB and SisDBP in DNA replication, DNA repair and DNA damage response, we constructed Sisssb and Sisdbp deletion mutants of S. islandicus. We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different cells, the wild type strain E233S, delta-Sisssb and delta-Sisdbp.