Proportion of GFP-positive MPs in the different MP subclasses, and GFP-CFTR mRNA content (a).
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(a)The proportion of GFP-positive MPs was determined by flow cytometry, and the GFP-CFTR mRNA content by qRT-PCR analysis. Data shown in the Table are mean values (m) ± SEM (n = 3).(b)The values obtained after RT-PCR amplification of a RNA fragment of 196 nt in length overlapping the GFP C-terminal and CFTR N-terminal sequences were expressed as ng RNA per 107 MP30 or MP100.
创建时间：
2015-12-02
