Decoding transcriptomic signatures of Cysteine String Protein alpha-mediated synapse maintenance

Cysteine string protein α (CSPα), encoded by the Dnajc5 gene, is a synaptic vesicle chaperone that is linked to synapse maintenance and neurodegeneration. To investigate the transcriptional changes associated with synapse maintenance, we performed single nucleus transcriptomics on the cortex of CSPα knockout (KO) mice and littermate controls. Nuclei was isolated from CSPα KO and littermate WT mice. DEGs, GO, RNA velocity and CellchatDB analysis were performed for the WT and KO samples.