Gene expression profiles in control, Nanos2-KO and Ddx6-KO murien germ cells.. Mus musculus

In embryonic male germ cells, an RNA-binding protein NANOS2 recruits its target RNAs to processing bodies (P-bodies), where they are repressed. This process is critically important to promote male-type germ cell differentiation. However, it remains unclear whether all NANOS2 functions depend on P-bodies. We revealed that essential gene for P-body assembly, Ddx6- and Nanos2- null germ cells exhibited both similar and distinct defects. To more comprehensively examine the difference between each KO, we carried out RNA-Seq analysis for each KO germ cells.