Trypanosome Isolates and Amplification Results

JE samples are from Uganda and were processed using Sigma Genomic DNA extraction kit, followed by precipitation with 3 M sodium acetate and absolute alcohol. TMRS samples were from Tanzania and the DNA was prepared using Qiagen DNA extraction kit. aSubgenus Trypanozoon PCR test [7]. bT. b. rhodesiense PCR test [3]. cT. b. gambiense PCR test [17]. dSource: Wendy Gibson, University of Bristol, UK. eThe samples were processed using the saponin lysis method [5]. fDNA prepared directly from human blood. gDNA prepared directly from human CSF. hSource: Philippe Büscher, Institute of Tropical Medicine Antwerp, Belgium. DNA prepared with Qiagen DNA extraction kit. nd, not done in this study (identification reported [17]).