Transcriptome of Arabidopsis thaliana seedlings treated with trans-Zeatin-N-glucosides

Ten-day old (10dag) Arabidopsis seedlings were floated in buffer (3mM MES pH 5.7) for two hours. Buffer solutions contained either 1uM trans-Zeatin (tZ), 1uM trans-Zeatin-7-glucoside (tZ7G), 1uM trans-Zeatin-9-glucoside (tZ9G), or 0.1% DMSO as a negative control. At least five seedlings were included per treatment. Three replicates of each treatment were performed. RNA was isolated using Qiagen RNeasy Plant Mini Kit. Library preparation and sequencing were performed by Novogene, Co. Transcriptome analysis was performed to determine if transcriptional effects of tZ7G and tZ9G mimicked transcriptional effects of the known active cytokinin tZ.