ZFP57 dictates allelic expression switch of target imprinted genes

We did RNA-seq analysis to compare gene expression profiles in mouse embryos with or without ZFP57. We also perfromed whole-genome bisulfite sequencing analysis (WGBS) for the Zfp57 mutant mouse embryos and control heterozygous embryos. We analyzed DNA methylation at the imprinting control regions (ICRs), differentially methylation regions (DMRs) and repetitive elements including LINE-1, LINE-2, SINE, LTR and IAP. Overall design: RNA-seq analysis was used to find the target genes of Zfp57 in mouse embryos. WGBS was applied to examine the effects of ZFP57 on DNA methylation at the ICRs, DMRs and some repetitive elements.