Glycolysis-derived protein lactylation orchestrates an immunosuppressive microenvironment to block pancreatic cancer immunotherapy

Integrated transcriptomic, proteomic and lactylomic profiling was performed on tumor specimens from 48 PDAC patients treated with adjuvant anti-PD-1-based therapy.Perform proteomic analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS). Identification of differentially expressed proteins (DEP) using fold change (FC) and p-value threshold, followed by bioinformatics analysis including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment.