Tumors positive for melanoma markers S100 and DCT.

In order to distinguish melanoma from non-melanoma tumors were subjected to staining with S100 and DCT (see Figure 1E, Figure S1). Only those stained positive for both markers were considered as melanomas. Number of positive stained out of total melanomas is shown (percent positive) for each of the genotypes. Statistical analysis using Chi-square assay revealed that the differences between # of melanomas between the ATF2−/− and ATF2+/+ genotypes are significant (p = 0.018). Differences among the other groups were not significant (differences between # of melanomas between the ATF2−/+ and ATF2+/+ p = 0.36; differences between # of non-melanomas between the ATF2−/− and ATF2+/+ groups p = 0.37. differences between # of non-melanomas between the ATF2−/− and ATF2−/+ p = 0.92).