UVB-induced skin autoinflammation due to NLRP1 mutation and its inhibition by anti-IL-1b antibody

To analyze the pathogenic mechanism of the autoinflammatory skin syndromes caused by an NLRP1 mutation, we sought to generate Nlrp1b mutation knock-in mice mice as a mouse model of cutaneous autoinflammatory lesions due to NLRP1 mutants, and examine the expression of proteins, such as cytokines, and the mRNA expression profile, including inflammasome-related genes in the model lesions. RNA sequencing was performed using extracted RNA from the skin of 5-week-age Nlrp1b knock in (KI) mice (homo and hetero) and wild type (WT) mice before and after UVB irradiation. We performed a gene set enrichment analysis using the hallmark gene set database.