Genome and developmental transcriptome of the forensically important blowfly, <em>Phormia Regina</em>
收藏资源简介：
Forensic entomologists often rely on the morphological traits of insects, such as length and weight, to estimate the time of death. Blowflies like Phormia regina are particularly significant in North American forensic investigations. However, age estimation of older P. regina maggots becomes challenging due to limited morphological changes during the lengthy L3 larval phase of development. To address this gap, we used transcriptomic profiling of blowfly maggots to generate molecular markers that specify their age. We first characterized maggot weight, behavior, and mRNA in 10-hour increments during development. At 27.5°C, the weight of the maggots increased from when first recorded at 70 through 100 hours and then remained stable from 110 hours to pupation. The behavioral transition between the feeding and the post-feeding wandering stage usually took place between 100 and 120 hours. Second, we built a chromosomal-scale Phormia regina genome annotated with long mRNA reads to provi..., Identifying the transition between feeding and wandering behavior We maintained a laboratory colony of a highly inbred line of Phormia regina. The source was from Dr. Amanda Roe at University of Nebraska at Lincoln [1]. Developmental rate data were available [1] . All rearing containers were maintained at 27.5+ 0.5 °C. under 16:8 light:dark cycle in one SMY04-1 DigiTherm CirKinetics Incubator (TriTech Research, Inc., Los Angeles, California, USA). The incubator was equipped with uniform lighting, additional fans, and a port for thermometer access. Each equal-age cohort was reared within a plastic 72 x72x100 mm insect breeding box (Gyeonggi-do, Korea). Each box contained 0.5cm sawdust and a 30g of fresh chicken liver in a suspended 4 oz paper cup. Eggs were obtained on a paper towel soaked with chicken liver blood placed in a cage of P. regina adults for 30 minutes before removal. The removal time was considered age zero for those insects. About 500-1000 newly deposited eggs were placed ..., , # **Genome and developmental transcriptome of the forensically important blowfly, *Phormia regina*** Dataset DOI: 10.5061/dryad.w6m905r04 **Description of the data and file structure** [https://doi.org/10.5061/dryad.w6m905r04](https://doi.org/10.5061/dryad.w6m905r04) Blowflies from the Calliphoridae family are drawn to decaying organic matter. Female blowflies lay eggs on corpses, which hatch into larvae within hours to a day. These larvae grow through several stages before becoming adults, aiding in estimating the postmortem interval based on their development stage. Forensic entomologists use traits like length and weight for time of death estimation, but older larvae pose challenges due to limited morphological changes during their lengthy L3 phase. Our dataset aims to estimate time of death by identifying genes in aging maggots of *Phormia regina*. We characterized maggot development and identified a transition between feeding and wandering stages at 100-120 hours at 27.5°C. We...,
法医昆虫学家常借助昆虫的形态学特征（如体长、体重）推断死亡时间。在北美法医调查中，亮蓝扁丽蝇（*Phormia regina*）具有尤为重要的应用价值。然而，在漫长的三龄（L3）幼虫发育阶段，形态变化极为有限，导致对高龄*P. regina*幼虫的虫龄估算极具挑战。为填补这一研究空白，本研究通过对丽蝇幼虫进行转录组分析，获得了可精准标定其虫龄的分子标记。研究首先以10小时为间隔，对发育过程中的幼虫体重、行为及mRNA表达进行了系统表征。在27.5℃条件下，幼虫体重自首次记录的70小时起至100小时持续增长，随后从110小时至化蛹阶段保持稳定。取食阶段与取食后扩散阶段的行为转换通常发生在100~120小时之间。第二，本研究构建了基于长mRNA读段注释的染色体水平*Phormia regina*基因组，以用于…… **取食与扩散行为的转换鉴定** 本研究维持了一株高度近交的*Phormia regina*实验室种群，其种源来自美国内布拉斯加大学林肯分校的Amanda Roe博士[1]，该种群的发育速率数据已公开[1]。所有饲养容器均置于一台SMY04-1型DigiTherm CirKinetics恒温培养箱（TriTech Research公司，美国加利福尼亚州洛杉矶）中，培养条件设置为27.5±0.5℃、16:8光暗周期。该培养箱配备均匀照明系统、辅助风扇及温度计接入端口。 同龄幼虫群均饲养于尺寸为72×72×100mm的塑料昆虫饲养盒（韩国京畿道生产）中，每盒内放置0.5cm厚的木屑，并在悬挂的4盎司纸杯中装入30g新鲜鸡肝作为食源。研究人员将浸有鸡肝浸出液的纸巾置于*P. regina*成虫笼中30分钟以获取虫卵，移除纸巾的时间即记为该批幼虫的虫龄零点。随后将约500~1000枚新产下的虫卵放置于…… # **具法医重要性的亮蓝扁丽蝇（*Phormia regina*）的基因组与发育转录组** 数据集DOI：10.5061/dryad.w6m905r04 **数据与文件结构说明** [https://doi.org/10.5061/dryad.w6m905r04] 丽蝇科（Calliphoridae）昆虫常被腐坏的有机质吸引，雌蝇会在尸体上产卵，虫卵可在数小时至一天内孵化成幼虫。幼虫历经多个发育阶段后羽化为成虫，研究人员可通过其发育阶段推断死后间隔时间（PMI）。法医昆虫学家常借助体长、体重等特征估算死亡时间，但高龄幼虫在漫长的L3阶段形态变化有限，给虫龄估算带来了挑战。 本数据集旨在通过鉴定*Phormia regina*老龄幼虫中的差异表达基因以实现死亡时间估算。研究对幼虫发育过程进行了系统表征，并确定在27.5℃条件下，取食与扩散阶段的行为转换发生于100~120小时之间。……
