A shift in the host web occupancy of dew‐drop spiders associated with genetic divergence in the Southwest Pacific

Aim
We assessed the population genetic structure of the kleptoparasitic spider Argyrodes bonadea across the Southwestern Pacific islands. Our aim is to evaluate the impact of overseas distances and, in particular, the Kerama gap, as potential drivers of genetic differentiation. If no relationship exists, then we assume dispersal following adaptive change as alternative non-vicariant mechanism that generates divergence.

Location
Southwestern Pacific Islands.
Taxon
Argyrodes bonadea.
Methods
We used mitochondrial Cytochrome Oxidase 1 (CO1) gene sequences and Restriction Site-associated DNA Sequencing (RAD-seq) for our analyses.
Results
Two strongly supported lineages, an Amami-Okinawa Lineage (AOL) and an Austral-Asia Lineage (AAL), correspond to two separate clades, roughly divided by the Kerama Gap, in phylogenetic trees estimated here. However, species delimitation led to the interpretation of only a single species present. The AOL exhibits complex, geographically structur..., , Use FigTree v.1.4.3 to visualize the MCC tree, # Data from: Dispersal and host-driven genetic divergence: a phylogenomic case of Argyrodes bonadea (Araneae, Theridiidae)

## Data files

### 1. Specimen list

The list includes 112 samples used for CO1 and RAD-seq analyses. The areas covered by our sample collection include Palawan and Surigao, Philippines; Queensland in Australia; the major Ryukyu island groups of Yaeyama, Miyako (includes Irabu and Ikema), Okinawa, and Amami in Southern Japan; Taitung, Taiwan. Argyrodes bonadea were found co-inhabiting the webs of different hosts such as Nephila, Argiope, and Cyrtophora, Cyclosa, and as well as Nihonhimea as observed during the fieldwork from 2007 to 2020.

### 2. VCF

Stacks v2.0 was used to assemble the raw RAD-seq sequences then we merged the forward and reverse strands using Paired-End read merger. The paired strands were aligned to Argyrodes miniaceus genome draft sequenced via Hi-seq 2500 platform as the reference with the aid of a standard alignment program Burrows-Wheeler Al...,