Primers for RT-qPCR amplification used in this study.
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https://figshare.com/articles/dataset/_Primers_for_RT_qPCR_amplification_used_in_this_study_/1020030
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aDesigned by an automated search in Primer Premier 5 software. Parameters for each primer pair: G+C content = 40–60%, annealing temperature between 58° and 62°C, and no false priming.
bAmplicon size and specificity were checked using melt curve and 2% agarose gel electrophoresis (Figure S3).
cEfficiency % = 10(−1/slope) −1. R2 values of all relative standard curves were more than 0.989.
创建时间：
2014-05-08
