Differences in gene expression between ASP WT and ASPcKO tumors

To further examine the consequences of Pten loss in a Hedgehog driven, murine FN-RMS, we used microarrays to determine the transcriptomic differences between our aP2-Cre;SmoM2 FN-RMS model (Hatley et al. 2012, Cancer Cell) and aP2-Cre; SmoM2; Pten flox/flox FN-RMSs. Four ASP wild-type and four ASPcKO tumors were removed, flash frozen in liquid nitrogen and pulverized in a TissueLyzer. RNA was extracted with Qiagen's miRNeasy RNA Elution Kit.