Massively parallel engineering of persistent CAR-Ts [GEO_CLASH_Nextera]. Massively parallel engineering of persistent CAR-Ts [GEO_CLASH_Nextera]

To identify immune-related gene perturbations that enhance CAR-T cell persistence in in vitro and in vivo settings, we generated thousands of CAR-T variants using the CLASH platform and evaluated crRNA representation across time in in vitro and in vivo cancer models. Overall design: After genomic DNA was collected from CAR-Ts, designed primers near the cutting site and performed PCR to generate a sequenceable guide library. Raw counts were processed using custom bash and R scripts.