Quantitative polymerase chain reaction data describing target gene silencing in the invasive common reed Phragmites australis subsp. australis using a leaf cutting bioassay

Name: Quantitative polymerase chain reaction data describing target gene silencing in the invasive common reed Phragmites australis subsp. australis using a leaf cutting bioassay
Creator: Oak Ridge Institute for Science and Education; Louisiana State University; United States Geological Survey; Department of Biological Sciences, Louisiana State University; Environmental Laboratory, U.S. Army Research and Development Center; SIMETRI, Inc.
Published: 2026-04-23T10:16:47+08:00

U.S. Geological Survey2026-04-23 收录

植物基因沉默

入侵物种防治

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These data describe layered double hydroxide (LDH) mediated delivery of novel gene silencing agents (GSAs) in leaf tissue of the invasive common reed Phragmites australis ssp. australis. Double-stranded and hairpin RNAs were designed and synthesized to target paralogous genes coding for phytoene desaturase (PDS) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). A leaf cutting bioassay was developed to rapidly screen GSAs for effective gene silencing action. Reduced expression of PDS and EPSPS were then characterized using reverse transcription quantitative PCR (RT-qPCR), the results of which are presented herein. Related sequences describing target genes from experimental plants are available via the National Center for Biotechnology Information's GenBank database (accession IDs: PQ634570, PQ634571, PQ634572, PQ634573).

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Oak Ridge Institute for Science and Education; Louisiana State University; United States Geological Survey; Department of Biological Sciences, Louisiana State University; Environmental Laboratory, U.S. Army Research and Development Center; SIMETRI, Inc.

Quantitative polymerase chain reaction data describing target gene silencing in the invasive common reed Phragmites australis subsp. australis using a leaf cutting bioassay

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