Effect of dabigatran, argatroban, DAPA or hirudin on 125I-thrombin binding to fibrin clots.

Binding of 125I-thrombin to γA/γA- or γA/γ′-fibrin clots in the absence or presence of dabigatran, argatroban, DAPA, or hirudin was determined by measuring the amount of 125I-thrombin in the supernatants of compacted clots prepared by incubating γA/γA- or γA/γ′-fibrinogen with a catalytic amount of batroxobin. EC50 values were determined by nonlinear regression analysis and converted to Ki values by correction for Kd of 125I-thrombin for fibrin. Change denotes the maximal change in the percentage of bound 125I-thrombin compared with that in the absence of inhibitor.