Analysis of MLLT3 genomic distribution and related chromatin features in fetal liver hematopoietic stem-progenitor cells [ChIP-seq_cultured_FL-HSPC]

MLLT3 binds to TSS of genes active in FL-HSPC Overall design: ChIP analysis of sorted FL-HSPC, uncultured or cultured for 4, and K562 cells. This is an addition to the other experiments in the superseries. Sonicated chromatin (250,000-100,000 cells per IP) DNA was immunoprecipitated with antibodies against the indicated mark. DNA was amplified with Nugen Ovation V2 kit and sequenced in single end 50bp in multiplexed run on an Illumina HiSeq4000 instrument.