Gene expression profile at single cell level of crude umbilical cord blood (UCB) and OP9 pre-conditioned UCB.

Umbilical cord blood (UCB) transplantation shows pro-angiogenic effect and contributes to symptom amelioration in animal models of cerebral infarction. OP9 is a stromal cell line used as feeder cells to promote hematoendothelial differentiation of embryonic stem cells. We co-cultured UCB 24hr with OP9 (i.e. OP9 preconditioning) and investigated its change in angiogenic properties and underlying mechanisms. Single cell RNA sequencing showed prominent phenotypic shift toward M2 in monocytic fraction of OP9 pre-conditioned UCB. RBC depleted human UCB was plated on 5-cm Petri dishes at a density of 1x106 cells/mL with OP9 cells ( i.e. OP9 pre-conditioning ). After 24 h culture,adherent and non-adherent cells were subjected to scRNA seq. RBC depleted crude UCB cells were used as a control.