TrwC-mediated site-specific integration into different recipient plasmids.
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Integration frequencies expressed as integrants/donor. Integrants were confirmed by PCR amplification of a region of the cointegrate, as explained in Experimental Procedures. The recipient strain was DH5α.1DH5α λpir was used as an internal control for conjugation efficiency, expressed as transconjugants/donor.
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2015-12-02



