Any unwanted regions for primer design in sequences can be masked using a pair of "" to keep the sequence unchanged ()
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https://figshare.com/articles/dataset/Any_unwanted_regions_for_primer_design_in_sequences_can_be_masked_using_a_pair_of_""_to_keep_the_sequence_unchanged_()/83979/1
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Alternatively, the unwanted regions can be replaced with "Ns" (). The included region can be specified by one pair "{}" and only one included region can be masked.<b>Copyright information:</b>Taken from "BatchPrimer3: A high throughput web application for PCR and sequencing primer design"http://www.biomedcentral.com/1471-2105/9/253BMC Bioinformatics 2008;9():253-253.Published online 29 May 2008PMCID:PMC2438325.
提供机构：
Naxin Huo; Yaqin Ma
创建时间：
2011-12-31
