Proneural genes define ground state rules to regulate neurogenic patterning and cortical folding
收藏doi.org2025-01-15 收录
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This data-set contains uncropped western blot images for Han et al. 2021
Figure 2G: Western blots of co-immunoprecipitation (Co-IP) of Ascl1 with Flag-Neurog2 IP with or without DNase I treatment in vitro (X-ray film).
Figure 7B: Western blots of Notch intracellular domain (NICD) levels for FACS sorted E12.5 CD15+ pro-, Neurog2+, Ascl1+ and double+ cortical NPCs (X-ray film).
Figure 7C: Western blots of NICD levels in P0 control and split-Cre;Rosa-DTA cortices (Bio-rad gel doc with GelCapture MicroChemi 2.2.0.0 software).
本数据集收录了Han等人在2021年的研究中使用的未经裁剪的蛋白质印迹图像。图2G展示了Ascl1与Flag-Neurog2的共免疫沉淀(Co-IP)印迹,以及是否经过DNase I处理的体外实验结果(X射线胶片)。图7B呈现了通过流式细胞术分选的E12.5阶段CD15+前体细胞、Neurog2+、Ascl1+以及双阳性皮层神经祖细胞(NPCs)的 notch 细胞内结构域(NICD)水平印迹(X射线胶片)。图7C则展示了P0阶段对照组以及split-Cre;Rosa-DTA皮层的NICD水平印迹(Bio-rad凝胶成像系统,配备GelCapture MicroChemi 2.2.0.0软件)。
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