Gene expression profile of endothelial cells in a 3D-casted mold of a human aneurysm.

Because the IA lesion morphology is complex, the blood flow conditions loaded on endothelial cells in each part of the lesion in situ vary greatly. Overall design: We created a 3D-casted mold of the human unruptured IA lesion and cultured endothelial cells on this model, which was then perfused with culture media to model physiological flow conditions. Then, gene expression profiles of endothelial cells in each part of the IA lesion were analyzed.