Carbonate chemistry analyses of water collected from near Walpole, ME from zooplankton hatching experiments during 2011

<p>Previous version date: 2012.12.06</p>

<p>Updated files and metadata from J. Christensen sent on 2015.05.26:</p>

<p>Previous file has been removed and metadata has been updated to reflect new report content. The following text is an excerpt from the summary included in the <em>Technical Report #15-009 linked under 'Acquisition description':</p>

<p>Experiments were conducted to test whether reduced pH of the ocean waters would negatively affect the hatching success of several zooplankton species found in northern ocean waters. These experiments were performed at the Darling Marine Center of the University of Maine, using local seawaters (salinities &gt; 30). Each experiment consisted of incubating fresh zooplankton eggs in several 20L seawater tanks at constant temperature for periods of up to 6 days. Each tank was maintained at fixed pH, and each experiment consisted of several tanks which covered a range of pH values at and below that of natural seawater. The measure of hatching success was the number of nauplii which were produced from the total number of incubated eggs. During the incubation, waters were extracted to measure carbonate system parameters and other key concentrations (titration alkalinity, total carbon dioxide, salinity, nitrate plus nitrite, ammonium, inorganic phosphate, and dissolved silicate), which were used to calculate the real pH of seawater in the incubation tanks. In addition, a ten day cruise covering the Gulf of Maine was taken in 2012 for the purpose of determining the distribution of carbonate species and pH in these waters. Analysis of these chemistry samples was performed at the Green Eyes LLC laboratory.</p>