A fluorometric assay for high-throughput phosphite quantitation in biological and environmental matrices

Phosphite, the anion of phosphorus acid, is increasingly recognized as an important metabolite in the global biogeochemical phosphorus cycle and as a fungicide and fertilizer in agriculture. As such, methods for detecting phosphite quantitatively and selectively are critical to evidencing phosphorus redox chemistry. Here, we present a fluorescence-based assay for phosphite, based on the NAD+-dependent oxidation of phosphite by phosphite dehydrogenase and the subsequent reduction of resazurin to resorufin. With the application of a thermostable phosphite dehydrogenase, a medium-invariant analytical approach, and novel sample preparation methods, the assay is capable of rapid and accurate phosphite quantification with a 3 mm limit of detection in a wide array of biologically- and environmentally-relevant matrices, including bacterial and archaeal cell lysate, seawater, anaerobic digester sludge, and plant tissue. We demonstrate the utility of the assay by quantitating phosphite uptake in ..., , , # Data from: A Fluorometric Assay for High-throughput Phosphite Quantitation in Biological and Environmental Matrices

### Data Overview:

A collection of fluorescence data collected using the phosphite (Phi) assay method described in \"A Fluorometric Assay for High-throughput Phosphite Quantitation in Biological and Environmental Matrices\". Excel files contain the fluorescence output data from the Spark 10m microplane reader (Tecan), unless otherwise noted, including the instrument's operating conditions, and the organization of fluorescence readouts by sample ID. Fluorescence values are averaged in triplicate, then the sample containing no 17X-PTDH is subtracted from all average values. Every sample has at least 3 points on a standard addition curve ranging from 0-5 nmol of spiked Phi (excluding example assays which have a wider range). Red boxes with \"OVER\" were samples that saturated the detector and were not used for any analysis.

Other supplied data include absorbance measurements...