RNA m5C Methylation Mediated by Ybx1 Ensures Hematopoietic Stem and Progenitor Cell Expansion

Our research hypothesis posits that RNA m5C methylation, mediated by Ybx1, plays a critical role in ensuring the expansion of hematopoietic stem and progenitor cells (HSPCs). Our data demonstrate that Ybx1 regulates the stability of m5C-modified mRNAs, influencing key cellular processes such as the cell cycle and proliferation in HSPCs. Notably, we found that depletion of Ybx1 leads to enhanced mRNA decay of critical cell-cycle regulators, ultimately impairing HSPC expansion. The Western blot data presented here serve as a key piece of evidence supporting the role of Ybx1. These images correspond to the figures in our manuscript and are named according to their sequence in the article for ease of reference.