mRNA profiling of liver-specific RORa KO mouse liver

The retinoic acid receptor-related orphan receptor a (RORa) is a member of the NR1 subfamily of orphan nuclear hormone receptors. RORa is an important regulator of various biological processes, including cerebellum development, cancer and circadian rhythm. To determine molecular mechanism by which hepatic deletion of RORa induces obesity and insulin resistance, we performed global transcriptome analysis from high-fat diet (HFD)-fed RORa f/f and RORa LKO mouse liver tissues. This analysis provides insight into molecular mechanisms for RORa in high-fat-diet condition. mRNA expression profiles of RORa f/f and RORa LKO mice liver with control diet or high-fat-diet were examined by Illumina HiSeq 2500.视黄酸受体相关孤儿受体α（retinoic acid receptor-related orphan receptor a, RORα）属于孤儿核激素受体（orphan nuclear hormone receptors）NR1亚家族。RORα是多种生物学过程的重要调控因子，涵盖小脑发育、癌症发生与昼夜节律调控。为阐明RORα肝脏特异性敲除诱导肥胖与胰岛素抵抗的分子机制，本研究对高脂饮食（high-fat diet, HFD）喂养的RORα f/f及RORα肝脏特异性敲除（liver knockout, LKO）小鼠的肝脏组织开展了全转录组分析。该分析可为高脂饮食条件下RORα的分子作用机制提供研究视角。本研究通过Illumina HiSeq 2500测序平台，检测了正常饮食或高脂饮食喂养的RORα f/f及RORα LKO小鼠肝脏组织的mRNA表达谱。