Single-nuclei RNA-seq of the nucleus tractus solitarii and area postrema at for baseline and LPS-activated cell types

To identify LPS activated cell types in the NTS and AP we region we used single-nuclei RNA seq comparing baseline cell types and cell types labeled using the TRAP2 mouse line crossed to the INTACT mouse line after labeling during LPS treatment. Overall design: Nuclei were isolated using douce homogenization and stained for NeuN. FACS was used to isolate neuronal nuclei (NeuN+).