Sodium butyrate induced gene expression changes in the Madin-Darby bovine kidney epithelial cells

The Madin-Darby bovine kidney epithelial cells, when at approximately 50% confluence (during the exponential phase), were treated with 10mM sodium butyrate for 24h. Butyrate induced profound changes in gene expression related with multiple signal transduction pathways such as cell cycle control, apoptosis and extracellular matrix remodeling in this cell line Keywords: stress response, two class unpaired design Overall design: Six single channel hybridizations were performed (treated vs control), 3 replicates each.

Madin-Darby牛肾上皮细胞在约50%的细胞融合度（处于指数生长期）时，经10mM丁酸钠处理24小时。丁酸钠诱导了该细胞系中与多个信号转导通路相关的基因表达发生深刻变化，包括细胞周期调控、细胞凋亡和细胞外基质重塑等。关键词：应激反应，两类别非配对设计。总体设计：共进行了六次单通道杂交实验（处理组与对照组），每组均有三个重复。