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Single cell RNA-seq analysis of reprogrammed neurons [Patch-seq2]

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https://www.ncbi.nlm.nih.gov/sra/SRP318740
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Purpose of the study is to compare the transcriptional profiles of reprogrammed neurons from spinal cord-derived astrocytes, analyzed after electrophysiological recordings, and control astroctes. Overall design: MACS-sorted astrocytes, obtained from postnatal mouse spinalc cord, were transduced in vitro with Ascl1 and Neurog2. Reprogrammed cells were analyzed by electrophysiology ar 3-to-4 weeks, and then single cells were collected and subjected to SmartSeq2 amplification
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2021-08-12
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