RNAseq for photoperiodic after-effect

Soybean (Glycine max) is a typical short- day plant (SDP), but it has been widely cultivated in high-latitude long- day (LD) regions because of the development of early-maturing genotypes which are photoperiod-insensitive. However, some early-maturing varieties displayed significant responses for maturity under different day lengths but not for flowering, depicting an evident photoperiodic after-effect with a poorly understood mechanism. To identify the flowering-time related genes influenced by pre-flowering SD treatments, we identified differentially expressed genes (DEGs) by performing transcriptomic sequencing of the early-maturing variety Heihe27 (MG 0) at R1 and R3 under pre-flowering SD or LD and post-flowering LD conditions.