Cytotoxic investigation of new compounds from a poisonous consumable mushroom <i>Sarcosphaera coronaria</i> (Jacq.) J. Schröt. (Syn: <i>sarcosphaera crassa</i> (Santi) Pouzar)

<i>Sarcosphaera coronaria</i>, a poisonous mushroom safe for consumption when cooked properly, was investigated for cytotoxic metabolites against HeLa (cervical), HT-29 (adenocarcinoma), and MCF-7 (breast) cancer cell lines. Polarity-based extracts from raw and baked mushroom parts were screened, advancing acetone and methanol extracts for detailed mycochemical analysis. Four new compounds were isolated from the methanol extract, including three amides: <i>N</i>-dodecanoyl-<i>N</i>-(ethylsulfinyl) hexadecanamide (<b>1</b>), <i>N</i>-(1,3,4-trihydroxyoctadecan-2-yl) tetracosanamide (<b>2</b>), <i>N</i>-(1,3,4-trihydroxycosane-2-yl) tetracosanamide (<b>3</b>) and a 1,2-dilinolenoyl glycerol (<b>4</b>). Compounds <b>1</b> and <b>2</b> were obtained from the raw mushroom, while compounds <b>3</b> and <b>4</b> were isolated from the baked mushroom. Structural elucidation was achieved via spectroscopic techniques, including IR, 1D and 2D NMR, and MS. Compound <b>4</b> exhibited significant cytotoxic activity against MCF-7 and HeLa cancer cell lines with IC₅₀ values of 52.44 ± 2.1 and 71.51 ± 0.8 µg/mL, respectively, and showed moderate activity against the HT29 cancer line (IC₅₀: 86.85 ± 1.7 µg/mL). Compounds <b>2</b> and <b>3</b> exhibited similar moderate cytotoxic activity against HT29 (IC₅₀: 83.83 ± 1.5 and 84.27 ± 3.2 µg/mL), MCF7 (IC₅₀: 85.91 ± 2.1 and 86.41 ± 2.7 µg/mL), and HeLa (IC₅₀: 90.91 ± 3.3 and 93.74 ± 1.48 µg/mL µg/mL) cancer cell lines, respectively. Due to the presence of these bioactive components, the mushroom has the potential to be developed into a valuable source of nutraceuticals.

<i>冠状球囊霉（Sarcosphaera coronaria）</i>是一种需经正确烹饪方可安全食用的有毒蘑菇。本研究针对其针对HeLa（宫颈癌细胞）、HT-29（腺癌细胞）及MCF-7（乳腺癌细胞）癌细胞系的细胞毒性代谢物展开探究。研究人员对取自生菇体与烘烤菇体的极性提取物进行了筛选，并优选丙酮提取物与甲醇提取物开展深入的真菌化学分析。从甲醇提取物中分离得到4种全新化合物，其中包括3种酰胺类化合物：<i>N</i>-十二酰基-<i>N</i>-(乙基亚磺酰基)十六酰胺（化合物1）、<i>N</i>-(1,3,4-三羟基十八烷-2-基)二十四酰胺（化合物2）、<i>N</i>-(1,3,4-三羟基廿烷-2-基)二十四酰胺（化合物3），以及1种1,2-二亚麻酰甘油（化合物4）。化合物1与2取自生菇体，化合物3与4则分离自烘烤后的菇体。通过红外光谱（IR）、一维与二维核磁共振波谱（1D/2D NMR）以及质谱（MS）等光谱技术完成了所有化合物的结构解析。化合物4对MCF-7与HeLa癌细胞系表现出显著的细胞毒性活性，半数抑制浓度（IC₅₀）分别为52.44±2.1 μg/mL与71.51±0.8 μg/mL；同时对HT-29癌细胞系呈现中等强度的细胞毒性活性，其IC₅₀值为86.85±1.7 μg/mL。化合物2与3对HT-29、MCF-7及HeLa癌细胞系均表现出相近的中等细胞毒性活性，对应的IC₅₀值分别为：HT-29系83.83±1.5 μg/mL与84.27±3.2 μg/mL，MCF-7系85.91±2.1 μg/mL与86.41±2.7 μg/mL，HeLa系90.91±3.3 μg/mL与93.74±1.48 μg/mL。鉴于该菇类含有上述生物活性成分，其具备开发为极具价值的营养保健品原料的潜力。

搜集汇总

背景与挑战

背景概述

该数据集包含了对有毒食用蘑菇Sarcosphaera coronaria中新型化合物的细胞毒性研究。研究从该蘑菇的甲醇提取物中分离出四种新化合物，并测试了它们对多种人类癌细胞系的活性，其中一种化合物显示出显著的细胞毒性。这些发现表明该蘑菇具有作为营养药物来源的潜力。