Fungus Raw sequence reads. Fungus

To date, the extent to which rhizobium introduction influences fungal communities is unclear, with few interactions between rhizobium and fungal community members of soybean rhizospheres revealed. As hosts, this study included two parental soybean cultivars contrasting in nodulation (P1: low nodulation, P2: high nodulation), along with their low nodulation (LN1-4) and high nodulation (HN1-4) recombinant inbred lines (RILs) that also varied across markers for BNF (Biological Nitrogen Fixation). ITS1 region amplicon sequencing was used to investigate the structure of fungal communities in bulk soils and in rhizospheres of the two parental soybean lines and their RILs grown under rhizobium inoculation and non-inoculation conditions, in order to reveal the potential complex interactions among rhizobia, soybean plants and fungal community.