Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo (lungs)

Here we compare gene expression profiles between lungs taken from a Wild type mouse to lungs from a lincRNA-Cox2 knockout mouse The aim of the experiment is to determine what impact lincRNA-Cox2 has on gene expression within the lung Lungs were extracted from 4 months old wild type and lincRNA-Cox2 knockout mice after CO2 euthanasia and cervical neck dislocation, snap frozen, homogenized in liquid nitrogen and resuspended in Trizol.本研究对比了野生型（Wild type）小鼠与长链基因间非编码RNA-Cox2（lincRNA-Cox2）敲除小鼠的肺组织基因表达谱。本实验旨在阐明lincRNA-Cox2对肺组织内基因表达的调控作用。实验中，研究人员对4月龄的野生型及lincRNA-Cox2敲除小鼠实施二氧化碳安乐死与颈椎脱臼处死，摘取肺组织后快速冷冻，于液氮中研磨匀浆，并重悬于Trizol试剂中。