RNAseq Data of Neural Stem Cells, Glial progenitors and Sox1 positive Glial progenitors

With this study we aimed to characterize the changes invoked in glial progenitor cells by activation of the endogenous copy of the master transcription factor Sox1. For this study, a progenitor cell line was derived from ES cells carrying a heterozygous GFP knock-in in the Sox1 ORF. Sox1 positive cells were identified by GFP expression, and separated by FACS. As control population, Sox1 negative progenitors were used, as well as GFP sorted and unsorted, Sox1 positive Neuroepithelial cells derived from the same ES cell line. Overall design: Samples were divided in four groups: Baseline (=Sox1 negative) glial progenitors, neural stem cells (unsorted neuroepithelial cells,NSCs), neural rosettes (GFP sorted neuroepithelial cells, NR), and Sox1 positive glial progenitors. For each group, three independent biological samples were generated on different days, resulting in a total of 12 samples;