SMG1 does not function through p53 to affect α-synuclein.

Shown are results wherein SMG1 and p53 were knocked down either individually or in combination to assess effects on α-synuclein. Westerns testing α-synuclein levels in these knockdown experiments show that SMG1 mediated activation of p53 is not involved in the increases in α-synuclein. Expression levels of α-synuclein for each siRNA condition normalized to the non-silencing (NS) siRNA control are shown beneath the total synuclein (α-tSyn) western. Antibodies used were as in Figure 3, with anti-p53 antibody (α-p53) included. siRNAs used are indicated across the top of the westerns, which are representative of three independent replicates.