Characterization of native protein complexes and protein isoform variation using size-fractionation-based quantitative proteomics.

Data from ProteomeXchange, PXD ID: PXD001220. File: PT1541S1F02.mzml. Published as part of Mol Cell Proteomics. 2013 Dec;12(12):3851-73 . From the Abstract: {{i}} Proteins form a diverse array of complexes that mediate cellular function and regulation. A largely unexplored feature of such protein complexes is the selective participation of specific protein isoforms and/or post-translationally modified forms. In this study, we combined native size-exclusion chromatography (SEC) with high-throughput proteomic analysis to characterize soluble protein complexes isolated from human osteosarcoma (U2OS) cells. {{/i}}