A volatile cue from a specialist herbivore primes gene expression against biotic stress in tall goldenrod (Solidago altissima L.) [Supporting information]

This repository contains scripts and data (i.e., jasmonic acid, salicylic acid, and abscisic acid) from the leaves of tall goldenrod (Solidago altissima) that were fed on by corn earworm (Helicoverpa zea, Lepidoptera: Noctuidae). In our study, we exposed S. altissima plants to the volatile emission of male goldenrod gall flies (Eurosta solidaginis), which primes their chemical defense in preparation for future attack. We then subjected plants to herbivory from corn earworm for 48 hours. We sampled multiple time points to gauge plant chemical defense at various times across sustained herbivory with and without prior exposure to the threat cue. Leaves sampled in this experiment were processed both for phytohormone measurement and RNA-seq; the sequencing reads for this study are available on the NCBI Sequence Read Archive under BioProject ID PRJNA1295474.